July 16, 2017

Download Advances in Clinical Chemistry, Vol. 19 by Oscar; Latner, A. L. Bodansky PDF

By Oscar; Latner, A. L. Bodansky

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Extra info for Advances in Clinical Chemistry, Vol. 19

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28 D. MOSS 0-5 6-10 O/o 11-15 16-20 Difference FIG. 12. Histogram of the agreement between duplicate estimations of lactate dehydrogenase activity in serum by a manual spectrophotoinetric method at 25°C (open bars) and by the LKB 8600 reaction-rate analyzer at 35°C (filled bars). Differences between duplicates are expressed as a percentage of the mean value for each pair. 05'C (Al). The 24 readings collected from each of the 24 cuvettes within a 2-minute period are entered into the 16K memory of a Nova 1200 computer.

The photometer cuvette is constructed of silver, to accelerate temperature equilibration, and has a path length of 1 cm. The volume of solution 44 D. W. 19. (A) The Perkin-Elmer KA-150 Kinetic Analyzer. (B) Diagram of operational sequence. ) required to fill it is 20 pl. Air-segmentation of the reaction mixture flowing through the cuvette is used to reduce carry-over from sample to sample. OS"C. Reaction mixtures corresponding to each sample reach the photometer cell 25 seconds after the reaction has been triggered by substrate.

W. MOSS 2000 - 1000 - - . 16. Correlation between alkaline phosphatase activity in serum (IU/liter at 37%) determined by the Beckman System T R analyzer and by the Technicon SMA multichannel analyzer. Both scales are logarithmic. mixture is carried round to a second dispensing position by the movement of the turntable, and the reagent is added which initiates the reaction. Stirring is effected by a rotating rod. The cuvette progresses into the light beam of the photometer. The absorbance reading is stored in a progranimable calculator, where it and subsequent readings on the same cuvette are used to compute catalytic concentration.

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