July 15, 2017

Download Animal Cell Technology: Basic & Applied Aspects: Proceedings by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji PDF

By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)

Animal mobilephone know-how is a becoming self-discipline of cellphone biology which goals not just to appreciate buildings, capabilities and behaviors of differentiated animal cells, but in addition to examine their talents for use for business and clinical reasons. The objective of animal telephone know-how comprises the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to supply proteins of scientific and pharmaceutical importantance, and the applying of animal cells to gene treatment, synthetic organs and the construction of practical meals. This quantity provides the readers an entire evaluation of the current cutting-edge and may be beneficial for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, rather mobilephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines relating to animal mobilephone culture.

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Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF

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Additional resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006

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IGF-1 and Insulin belong to the same family of structurally related acid peptides and so have similar tertiary structures [3]. LONG™ R3 IGF-1 was created for use in cell culture by substituting the third amino acid of IGF-1 with an arginine and adding a 13 amino acid N-terminal extension [2, 4]. LONG™ R3 IGF-1 is used to provide an inexpensive yet high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media. Most cell types secrete IGF binding proteins in various forms [2, 3], which inhibit the activity of IGF-1 by requisitioning the peptide and preventing interactions between IGF-1 and IGF-1 receptor [3].

Cells cryopreserved by sericin solution proliferated for 25 h after thawing, as did those preserved in FBS solution, but after 25 h, proliferation of the former was significantly superior to the latter. On the other hand, insulin production at day 3 after thawing was superior in cells preserved in FBS solution, but the difference was small (Fig. 1b). 44 T. Toyosawa et al. b 60 Secreted insulin concentration (ng/ml) Viable cell number (×104 cells) a 50 40 30 20 10 0 0 25 50 Culture period (hours) 100 600 500 400 300 200 100 0 FBS Sericin Cryopreservative solution Fig.

1–10 ng/ml, proliferation was enhanced. Figure 1b shows the effect of LIF. 1–1 ng/ml, proliferation was also increased, but at a concentration of 10 ng/ml, proliferation was inhibited. After adaptation to serum-free medium, the response to the cytokines was altered. Figure 2a shows that IL-6 suppressed the proliferation of 2E3-O cells adapted to serum-free medium. Figure 2b shows that LIF also inhibited the proliferation of 2E3-O cells. In the presence of IL-6 and LIF, the cells retained high viability despite their proliferation being strongly inhibited (Fig.

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