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Additional info for Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods
This detail is especially important for photography. In addition, an excess of liquid will prevent proper sealing of the cover slip in any attempt to make a permanent mount of the preparation. More enduring mounts can be produced by placing a small drop of lactophenol cotton blue on the cover slip bearing the fungal growth and covering it with a second cover slip of smaller size. This whole preparation is then placed with the smaller cover slip facing downward onto a drop of balsam or other permanent mounting material in the center of a slide.
Procedure 1. Place specimen on clean glass slide 2. Add a drop of each solution (15% KOH and Calcofluor white) 3. Mix and cover with glass cover slip. 4. Allow to sit at room temperature until material is clear (warming slide will hasten process). 5. Examine using a fluorescence microscope with 10X and 40X to 50X objective or 100X oil. Interpretation: Fungi stain bright green or blue-white, depending on the filter. 5 Recommendations for Isolation of Fungi from Ocular Specimens RECOMMENDED MEDIA FOR FUNGI ISOLATION Clinical specimens are processed promptly and plated to isolation media as a means to recover fungi that may be causing disease.
The conidia are bound by a gelatinous material. They may be single or multicellular, fusiform with a slight curve or resemble a shallow crescent. These structural properties of conidia vary depending on the species. • Acremonium falciforme usually produces crescentic, nonseptate conidia. Sometimes, 2 or 3 celled conidia may also be observed. 2). Macroscopic and Microscopic Characteristics of Ocular Fungal Isolates Fig. 2: Acremonium species. Oblong conidia accumulating at the apices of narrow Phialides 45 46 Aravind’s Atlas of Fungal Corneal Ulcers ALTERNARIA Pathogenicity Occasional agents of keratitis, other infections include onychomycosis, ulcerated cutaneous infection, chronic sinusitis and rare cases of deep infection have also been reported in the immunocompromised patient.
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